This info has been taken from the interactive gene tree
Accession codes of all genes for 13 taxa are available in a Microsoft Excel document
Genes either lost from B. saltans or gained by P.confusum
biopterin/folate/pteridine transporter
enzymes of ubiquinone biosynthesis
Cyclopropane-fatty-acyl-phospholipid synthase
methylthioadenosine phosphorylase
3-demethylubiquinone-9 3-methyltransferase
2-polyprenyl-6-hydroxyphenyl methylase
3-demethylubiquinol 3-O-methyltransferase or hexaprenyldihydroxybenzoate methyltransferase
2-octaprenyl-6-methoxyphenol hydroxylase or monooxygenase
ubiquinone biosynthesis methyltransferase
ubiquinone biosynthesis methyltransferase or 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
pteridine reductase PTR1
Bromodomain factor 1
farnesyl pyrophosphate synthetase or solanesyl diphosphate synthase
ferrous iron transport protein, putative (LIT1-1)
S-methylthioribose kinase
glyceraldehyde-3-phosphate dehydrogenase-like
Adenosine deaminase
tyrosine aminotransferase, putative
enoyl-CoA hydratase, enoyl-CoA isomerase/3-hydroxyacyl dehydrogenase(trifunctional enzyme, partial)
xylulokinase
The ancestral trypanosmatid lost over 50% of its genes relative to B. saltans, including the following metabolic genes:
2-amino-3-carboxymuconate-6-semialdehyde decarboxylase
3-hydroxyisobutyryl-coenzyme a hydrolase-like protein
aspartic protease (lysosomal)
Cathepsin D (aspartic protease, 3.4.23.5)
Galactokinase
glucoamylase-like protein
lysine ketoglutarate reductase| saccharopine dehydrogenase
Lysosomal aspartic protease
mitochondrial RNA helicase-like protein
N-acetylmuramate 6-phosphate etherase
ornithine aminotransferase
peroxisomal acyl-CoA oxidase
thiolase protein-like
trehalase
UDP-galactopyranose mutase
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
Genes acquired after the split Trypanosomatids and Paratrypanosoma
beta-hydroxybutyrate dehydrogenase
D-3-phosphoglycerate dehydrogenase
Haem uptake protein or multi-pass transmembrane protein (LHR1)
putative threonine dehydratase
Serine racemase (threonine dehydratase)
Ribulokinase
thiolase protein-like (most likely trifunctional protein Beta subunit), homologous to yeast peroxisomal fox3 but lacks PTS
threonine dehydratase
UDP-galactose /glucose pyrophosphorylase
xanthine-guanine phosphoribosyltransferase xprt
Genes lost after the split of paratrypanosoma and other trypanosomatids
3-hydroxyanthranilate-dioxygenase
Histidine-ammonia lyase
Imidazolonepropionase
Formiminoglutamase
kynurenine 3-monooxygenase
Tryptophan enzymes 1,3 and 5:
tryptophan-dioxygenase oxidoreductase, kynurenine monodioxygenase, 3-hydroxyanthranitae dioxygenase
urocanate hydratase
Genes lost in the split of Trypanosoma and (Blechomonas plus Phytomonas)
D-lactate dehydrogenase-like (glycosomal)
D-lactate dehydrogenase-like protein (glycosomal)
Methylmalonyl-CoA epimerase (racemase), mitochondrial
methylmalonyl-CoA mutase, putative
propionyl-CoA carboxylase
phosphatidylethanolamine n-methyltransferase-like protein
S-methylthioribose kinase
xylulokinase
Genes acquired by Trypanosoma
GPI inositol deacylase 2 | GPIdeAc2
phospholipase A1, putative
proline racemase
Genes lost from all Trypanosoma
4-coumarate--CoA ligase/fatty acyl-CoA synthase
C-22 sterol desaturase|cytochrome p450-like protein
chitinase
Cyclopropane-fatty-acyl-phospholipid synthase
D-3-phosphoglycerate dehydrogenase
methionine synthase, (Homocysteine S-methyltransferase), cobalamin dependent
methylenetetrahydrofolate reductase, putative
methylthioribulose-1-phosphate dehydratase/enolase-phosphatase E1 (bifunctional)
phenylalanine-4-hydroxylase, putative
serine hydroxymethyltransferase, cytosolic
serine hydroxymethyltransferase, mitochondrial
thiamine biosynthesis-like protein
xanthine-guanine phosphoribosyltransferase xprt
Genes acquired by the Leishmaniinae
arginase, putative
argininosuccinate synthase
Choline/Carnitine o-acyltransferase-like protein (probably cytosolic)
coproporphyrinogen III oxidase
D-3-phosphoglycerate dehydrogenase
dihydroxyacetone kinase (triose kinase)
Galactokinase
ferrochelatase
gamma-glutamyl kinase
glucoamylase-like protein
glutamate dehydrogenase (NADP), putative
Indole-3-pyruvate decarboxylase | phenylpyruvate decarboxylase
Kynurenine--oxoglutarate transaminase
L-2-hydroxyglutarate dehydrogenase
lipase
membrane-bound acid phosphatase, putative
methionine synthase, cobalamin independent
mitochondrial RNA helicase-like protein
old yellow enzyme | NADH:flavin oxidoreductase/NADH oxidase
protoporphyrinogen oxidase-like protein
UDP-galactopyranose mutase
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
Metabolic genes lost by the Leishmaniinae
alternative oxidase (TAO)
Arginine kinase
hydroxymethylglutaryl-CoA lyase
L-threonine dehydrogenase
sedoheptulose-1,7-bisphosphatase
Genes acquired by the African trypanosomes
Dicer protein DCL2
Genes lost from the African trypanosomes
2-aminoethylphosphonate:pyruvate aminotransferase-like protein
Acireductone synthase / enolase-phosphatase E1 (bifunctional) | 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
asparaginase-like protein|similar to
Asparaginase, putative
CMP-sialic acid transporter (CMP-Sia-Tr)
D-lactate dehydrogenase (homologous to yeast Dld3, acts on D-2-hydroxyglutarate
dihydroxyacetone kinase (triose kinase)
formiminoglutamase
fructosamine kinase, putative
FTHS (c-1-tetrahydrofolate synthase, cytoplasmic, putative)
Galactokinase
gamma-glutamyl phosphate reductase-like protein
glyoxalase I, trypanothione-dependent
Glucokinase
glutamate dehydrogenase (NADP), putative
Haem uptake protein or multi-pass transmembrane protein (LHR1)
histidine-ammonia lyase
imidazolonepropionase
L-ascorbate peroxidase
Na/H antiporter-like protein
old yellow enzyme | NADH:flavin oxidoreductase/NADH oxidase
phosphoenolpyruvate mutase
phosphoglucomutase
Phosphomannomutase-like
protein geranylgeranyltransferase type II alpha subunit
quinonoid dihydropteridine reductase (QDPR)
Ribulokinase
Serine acetyltransferase
short chain 3-hydroxyacyl-CoA dehydrogenase
thiol-dependent reductase TDR1 | Glutathione dehydrogenase (ascorbate)
thiolase protein-like (most likely trifunctional protein Beta subunit), homologous to yeast peroxisomal fox3 but lacks PTS
tyrosine aminotransferase, putative
UDP-galactopyranose mutase
UDP-glucuronate decarboxylase
Urocanase
Metabolic genes acquired by B. ayalai and the phytomonads
Isopropyl alcohol dehydrogenase | Alcohol dehydrogenase (Zn-containing) (1.1.1.1) (NAD)
Genes lost by both B. ayalai and the Phytomonads
beta-hydroxybutyrate dehydrogenase
Argonaute 1 AGO1
Choline/Carnitine o-acyltransferase-like protein (probably cytosolic)
Dicer protein DCL1
hydroxymethylglutaryl-CoA lyase
Kynurenine--oxoglutarate transaminase
L-2-hydroxyglutarate dehydrogenase
lipase
membrane-bound acid phosphatase, putative
methionine synthase, cobalamin independent
old yellow enzyme | NADH:flavin oxidoreductase/NADH oxidase
sedoheptulose-1,7-bisphosphatase
Genes acquired by crithidia and Leptomonas
Argininosuccinate lyase
Catalase
Cathepsin D (lysosomal aspartatic protease)
lysine/diaminopimelate decarboxylase
diaminopimelate epimerase
glycerol dehydrogenase, bacterial type
Genes lost by Crithidia and Leptomonas
Glyceraldehyde-phosphate dehydrogenase-like
Genes acquired by T.brucei and T.congolense
none
Genes lost from T. brucei and T. congolense
proline racemase
putative threonine dehydratase
Serine racemase (threonine dehydratase)
saccharopine dehydrogenase
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
metabolic genes acquired by the phytomonads
Cathepsin D (aspartic protease (lysosomal)
More than 1000 genes were lost by the phytomonads
2-aminoethylphosphonate:pyruvate aminotransferase-like protein
3-hydroxyisobutyryl-coenzyme a hydrolase-like protein
3-methylcrotonoyl-coa carboxylase beta subunit-like
4-coumarate--CoA ligase/fatty acyl-CoA synthase
acid phosphatase
acyl-coa dehydrogenase (long-chain specific),isovaleryl-coA dehydrogenase
acyl-coa dehydrogenase (medium chain specific)
acyl-coa dehydrogenase (short/branched chain specific)
acyl-coa dehydrogenase, putative (short branched chain)
aminomethyltransferase, or glycine synthase, mitochondrial precursor, putative (subunit of the glycine-cleavage system or T protein) (2 copies)
apocytochrome c1
aromatic L-alpha-hydroxyacid dehydrogenase
asparaginase-like protein
Asparaginase, putative
ATP synthase beta subunit, putative
Beta-ketoacyl (ACP) synthase family protein
3-oxoacyl-acyl carrier protein synthase II
C-22 sterol desaturase,cytochrome p450-like protein
chitinase
Cytochrome c oxidase assembly protein, putative
cytochrome c oxidase subunit 5, probable
cytochrome c oxidase subunit viii
cytochrome c oxidase subunit iv
cytochrome c oxidase subunit IX
cytochrome c oxidase subunit vi
cytochrome c oxidase subunit vii
D-3-phosphoglycerate dehydrogenase
D-lactate dehydrogenase (homologous to yeast Dld3, acts on D-2-hydroxyglutarate)
delta-6-fatty acid (linoleyl-CoA?) desaturase-like
electron transfer flavoprotein-ubiquinone oxidoreductase
enoyl-coa hydratase, mitochondrial
enoyl-coa hydratase, peroxisomal
enoyl-CoA hydratase/enoyl-CoA isomerase/3-hydroxyacyl dehydrogenase(trifunctional enzyme, partial)
Ferric reductase transmembrane protein (LFR1)
ferrochelatase-like protein
FTHS (c-1-tetrahydrofolate synthase, cytoplasmic, putative)
gamma-glutamyl phosphate reductase-like protein
glyoxalase I, trypanothione-dependent
Glucosamine-6-phosphate deaminase
Glucosamine-6-phosphate isomerase
glycerol-3-phosphate dehydrogenase, possible ER
glycine dehydrogenase (Glycine-cleavage complex P-protein)
glycosylphosphatidylinositol-specific phospholipase C
kynureninase, possible
L-ascorbate peroxidase
ascorbate peroxidase
L-gulonolactone oxidase, putative
L-threonine 3-dehydrogenase
lathosterol oxidase-like protein, C5 sterol desaturase
methionine synthase, (Homocysteine S-methyltransferase), cobalamin dependent
methylenetetrahydrofolate reductase, putative
MTFC/MTFD (C-1-tetrahydrofolate synthase, cytoplasmic, putative) bifunctional protein
Na/H antiporter-like protein
old yellow enzyme , NADH:flavin oxidoreductase/NADH oxidase
PEX12
PEX14, putative
phenylalanine-4-hydroxylase, putative
phospholipase A2 ?
phospholipase c-like protein
Phosphomannomutase-like
propionyl-coa carboxylase beta chain, putative
protein geranylgeranyltransferase type II alpha subunit
protoheme IX farnesyltransferase
putative electron transfer flavoprotein beta-subunit beta-etf-flavoprotein small subunit
pyruvate dehydrogenase (lipoamide) kinase,
quinonoid dihydropteridine reductase (QDPR)
reiske iron-sulfur protein precursor
Ribulokinase
sco1 protein homolog, mitochondrial precursor, probable
Serine acetyltransferase
short chain 3-hydroxyacyl-CoA dehydrogenase
thiolase protein-like (most likely trifunctional protein Beta subunit), homologous to yeast peroxisomal fox3 but lacks PTS
transaldolase, probable
ubiquinol--cytochrome-c reductase, probable
UDP-glucose pyrophosphorylase | UTP-glucose-1-phosphate uridylyltransferase 2
UTP-glucose-1-phosphate uridylyltransferase, UDP-glucose pyrophosphorylase
uracil phosphoribosyltransferase
Genes acquired by the Leptomonads
none
Genes lost by the Leptomonads
aromatic L-alpha-hydroxyacid dehydrogenase
fructosamine kinase, putative
Only in T. cogolense
none
Genes lost from T. congolense
3-ketoacyl-coa thiolase, possible
cdp-diacylglycerol synthetase-like protein
cystathionine beta-synthase, probable
cysteine synthase
delta-6-fatty acid (linoleyl-CoA?) desaturase-like,
Diphosphomevalonate decarboxylase
frataxin
farnesyl pyrophosphate synthetase|solanesyl diphosphate synthase
glucoamylase-like protein
glyceraldehyde-3-phosphate dehydrogenase-like
glycine-cleavage system H protein, putative
LABCG5 ABC transporter of haem
Mannose-1-phosphate guanyltransferase | GDP-D-mannose pyrophosphorylase
N18M|20 kDa subunit
riboflavin kinase/fmn adenylyltransferase-like protein
pterin-4-alpha-carbinolamine dehydratase, putative
putative threonine dehydratase
ubiquinol--cytochrome-c reductase, probable
Only in T. brucei
none
Genes lost from T. brucei
putative threonine dehydratase
Only acquired by T. vivax
putative threonine dehydratase
Serine racemase (threonine dehydratase)
saccharopine dehydrogenase
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
Genes lost by T. vivax
3-demethylubiquinone-9,3-methyltransferase
2-polyprenyl-6-hydroxyphenyl methylase
3-demethylubiquinol 3-O-methyltransferase/ hexaprenyldihydroxybenzoate methyltransferase
3-oxoacyl-(acyl-carrier protein) reductase
6-phosphogluconolactonase, putative | gulonolactonase
cystathionine gamma-lyase
delta-1-pyrroline-5-carboxylate dehydrogenase, putative
frataxin
homoserine kinase
L-gulonolactone oxidase, putative
LABCG5 ABC transporter of haem
pterin-4-alpha-carbinolamine dehydratase, putative
Threonine synthase-like
UDP-glucose pyrophosphorylase | UTP-glucose-1-phosphate uridylyltransferase 2
UTP-glucose-1-phosphate uridylyltransferase / UDP-glucose pyrophosphorylase
uracil phosphoribosyltransferase
Genes acquired by T. cruzi
alanine racemase
Genes lost from T. cruzi
ACPM|SDAP Acyl carrier protein
alternative oxidase (TAO)
aromatic aldehyde dehydrogenase, mitochondrial precursor (10-formyl-THF dehydrogenase-like)
argonaute protein AGO1
ATP synthase delta chain, mitochondrial precursor-like protein
ATP synthase epsilon (or delta) subunit, possible
Dicer protein DCL1
glutamate dehydrogenase (NAD)
glycerol-3-phosphate dehydrogenase (NAD)
methionine synthase, cobalamin independent
mitochondrial carrier protein-like protein (uncoupling protein)
mitochondrial RNA helicase-like protein
ornithine decarboxylase, probable
PEX10 | peroxisome assembly protein, putative
PEX2 |glycosome import protein (GIM1)
Phosphatidylserine decarboxylase proenzyme-like protein
phosphatidylserine synthase-like
phospholipase c-like protein
Phosphopantothenate--cysteine ligase (EC 6.3.2.5)|(PPC synthetase)
ribokinase-like protein
Ribonucleoside-diphosphate reductase (ribonucleotide reductase)
S-adenosylmethionine decarboxylase (regulatory subunit, prozyme)
Genes acquired by B. ayalai
gamma-glutamyl phosphate reductase-like protein
ferrochelatase-like protein
Genes lost from B.ayalai
alternative oxidase (TAO)
argininosuccinate synthase
Choline/Carnitine o-acyltransferase-like protein (probably cytosolic)
coproporphyrinogen III oxidase
dihydroxyacetone kinase (triose kinase)
Galactokinase
gamma-glutamyl kinase
glucoamylase-like protein
glutamate dehydrogenase (NADP), putative
Indole-3-pyruvate decarboxylase | pyruvate/indole-pyruvate carboxylase
iron/ascorbate oxidoreductase family protein, putative or thymine-7-hydroxylase-like protein
Kynurenine--oxoglutarate transaminase
L-2-hydroxyglutarate dehydrogenase
lipase
methionine synthase, cobalamin independent
mitochondrial RNA helicase-like protein
old yellow enzyme | NADH:flavin oxidoreductase/NADH oxidase
protoporphyrinogen oxidase-like protein
thiol-dependent reductase TDR1 | Glutathione dehydrogenase (ascorbate)
UDP-galactopyranose mutase
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
Genes acquired by strain EM1
Galactokinase
Genes lost by Phytomonas EM1
GMP synthase
iron-sulfur cluster NifU-like protein
ornithine decarboxylase
phosphatidylethanolamine n-methyltransferase-like protein
Phosphopantothenate-cysteine ligase
putative proton-translocating inorganic pyrophosphatase
vacuolar-type proton translocating pyrophosphatase 1
PYR2 aspartate carbamoyltransferase
PYR3 dihydroorotase (fragment)
Succinate dehydrogenase [ubiquinone] iron-sulfur subunit
UDP-galactose /glucose pyrophosphorylase (2.7.7.64)
Genes acquired by strain Hart1
Indole-3-pyruvate decarboxylase | pyruvate/indole-pyruvate carboxylase
Genes lost by Phytomonas Hart 1
Acireductone synthase/enolase-phosphatase E1 (bifunctional) | 2,3-diketo-5-methylthio-1-phosphopentane phosphatase
C-14 sterol reductase, putative
Cyclopropane-fatty-acyl-phospholipid synthase
dihydroxyacetone kinase (triose kinase)
fructose-6-phosphate2-kinase/fructose-2,6-bisphosphatase
Glucokinase
GPI mannosyltransferase
Haem uptake protein or multi-pass transmembrane protein (LHR1)
Inosine-adenosine-guanosine-nucleosidehydrolase|IAGNH
membrane-bound acid phosphatase precursor MBPA
methionine synthase reductase, mitochondrial precursor-like
MTFC/MTFD(C-1-tetrahydrofolate synthase, cytoplasmic, putative) bifunctional protein
N18M|20 kDa subunit
Phosphatidylserine decarboxylase proenzyme-like protein
Phosphomannomutase, putative
pterin-4-alpha-carbinolamine dehydratase, putative
Sterol 14-alpha (lanosterol) demethylase (CP51)
ycf45 chloroplast homologue
Genes acquired by L. seymouri
none
Metabolic genes lost by L. seymouri
4-coumarate--CoA ligase/fatty acyl-CoA synthase
Alanine aminotransferase
argininosuccinate lyase
argininosuccinate synthase
argonaute protein AGO1
ATP synthase alpha subunit
ATP synthase alpha subunit
coproporphyrinogen III oxidase
diaminopimelate epimerase
beta-glucosidase tr07374,tr07375,tr07376
Dicer protein DCL1
Ferric reductase transmembrane protein (LFR1)
iron/ascorbate oxidoreductase family protein, putative or thymine-7-hydroxylase-like protein
lathosterol oxidase-like protein,C5 sterol desaturase
lipase
lysine/diaminopimelate decarboxylase tr08303|g08888_seq27|g08889_seq27
Maltase Glucoamylase (Alpha-glucosidase) () (3.2.1.3) (Glucan-1,4-alpha-glucosidase)
alpha-glucosidase
membrane-bound acid phosphatase precursor MBPA
Monoglyceride lipase
Phosphoenolpyruvate carboxykinase [ATP], glycosomal
phosphofructokinase
s-adenosylmethionine synthase or methionine adenosyltransferase
thiol-dependent reductase TDR1 | Glutathione dehydrogenase (ascorbate)
Genes acquired by L. pyrrhocoris
none
Genes lost from L. pyrrhocoris
aminomethyltransferase, or glycine synthase, mitochondrial precursor, putative (subunit of the glycine-cleavage system or T protein)
citrate synthase
glycerol-3-phosphate dehydrogenase, possible ER
pterin-4-alpha-carbinolamine dehydratase, putative
Genes acquired by C. fasciculata
none
Genes lost from C. fasciculata
aminomethyltransferase, or glycine synthase, mitochondrial precursor, putative (subunit of the glycine-cleavage system or T protein)
citrate synthase
glycerol-3-phosphate dehydrogenase (NAD)
glycerol-3-phosphate dehydrogenase, possible ER
Phosphopantothenoylcysteine decarboxylase (EC 4.1.1.36) (PPC-DC) (CoaC)
protein geranylgeranyltransferase type II alpha subunit
pteridine reductase
Genes acquired by L. major relative to C. fasciculata and L.pyrrhocoris
ATP synthase beta subunit, putative
glyceraldehyde-3-phosphate dehydrogenase-like
glycerol-3-phosphate dehydrogenase, possible ER
ubiquinol--cytochrome-c reductase, probable
Genes lost from L. major
argonaute protein AGO1
beta-hydroxybutyrate dehydrogenase
Cyclopropane-fatty-acyl-phospholipid synthase
Dicer protein DCL1
glycosylphosphatidylinositol-specific phospholipase C
S-methylthioribose kinase
Metabolic genes lost at by P. confusum only
1-acyl-sn-glycerol-3-phosphate acyltransferase-like protein
3-hydroxyisobutyryl-coenzyme A hydrolase-like protein
3'-nucleotidase
alternative oxidase (TAO)
glutathione (trypanothione) peroxidase
adenylosuccinate lyase
arginine kinase
ATP synthase beta subunit, putative
gamma-glutamyl phosphate reductase-like protein
Glucosamine-6-phosphate isomerase
Glucosamine-6-phosphate deaminase
glutathione (trypanothione) peroxidase
methionine synthase, cobalamin independent
D-3-phosphoglycerate dehydrogenase
PEX10 | peroxisome assembly protein, putative
PGFS||prostaglandin f2-alpha synthase|Large subunit of NADP+ dependent arabinose dehydrogenase| reductasepyruvate carboxylase
phenylalanine-4-hydroxylase, putative
PGFS,prostaglandin f2-alpha synthase,Large subunit of NADP+ dependent arabinose dehydrogenase/reductase | aldoketoreductase
quinonoid dihydropteridine reductase (QDPR)
Phosphopantetheine adenylyltransferase,hypothetical protein
Genes either lost from B. saltans or gained by P.confusum
biopterin/folate/pteridine transporter
enzymes of ubiquinone biosynthesis
Cyclopropane-fatty-acyl-phospholipid synthase
methylthioadenosine phosphorylase
xylulokinase
3-demethylubiquinone-9 3-methyltransferase
2-polyprenyl-6-hydroxyphenyl methylase
3-demethylubiquinol 3-O-methyltransferase or hexaprenyldihydroxybenzoate methyltransferase
2-octaprenyl-6-methoxyphenol hydroxylase or monooxygenase
ubiquinone biosynthesis methyltransferase
ubiquinone biosynthesis methyltransferase or 2-methoxy-6-polyprenyl-1,4-benzoquinol methylase
pteridine reductase PTR1
Bromodomain factor 1
farnesyl pyrophosphate synthetase or solanesyl diphosphate synthase
ferrous iron transport protein, putative (LIT1-1)
S-methylthioribose kinase
glyceraldehyde-3-phosphate dehydrogenase-like
Adenosine deaminase
tyrosine aminotransferase, putative
enoyl-CoA hydratase, enoyl-CoA isomerase/3-hydroxyacyl dehydrogenase(trifunctional enzyme, partial)